Askion

Askion

Your partner from initial idea up to series production

content

Cryobiotechnology

Contact person
Dr. Egon Pfeifer
egon.pfeifer@askion.com
Phone: +49 (0)365 7353 200
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FAQ


1. What advantages does the system over conventional systems?


  • a closed cooling chain
  • minimal ice formation
  • PC controlled tracking system for sample and process data
  • semi-automated probe handling with motorized lift system
  • customized freezer system
  • open and modular system for probe formats and storage capacity
  • configurable software interface to different LIS/ HIS systems
  • error minimization by SOP based/supported user guide (Standard Operating Procedure)
  • automated logfile writing
  • built-in emergency handling


2. Which sample formats can be processed in general?


  • vials of all sizes and vendors (e.g. NALGENE, NUNC, SARSTEDT, BRAND 1…5ml with internal or external thread)
  • vials with integrated memory chip “ICEBREAKER“ by PERMA CRYO TECHNOLOGIE (www.perma-cryo.com)
  • blood bags in cassettes of all common vendors (e.g. MILTENYI, FRESINIUS DF200, MACOPHARMA, BAXTER) sizes up to 200 mm length (conforms with 100 ml filling volume)
  • straws (e.g. CRYOBIOSYSTEM)


3. What are the advantages of samples with electronic memory chips?


  • firm connection between the sample and data
  • captive position of the samples inside the cryo tank
  • logfile writing
  • fast inventory of the storage tank
  • easy local reading of sample data without access to the external database
  • prevention of falsifying samples


4. What formats can be equipped with electronic memory chips?


  • All common storage systems can equip with electronic memory chips. Available are adapters for tubes (ca. 1-2ml) from common vendors. (e.g. NUNC, GREINER, SARSTEDT)


5. What are the advantages of storage in the gas phase above liquid nitrogen?


  • no risk of samples contamination
  • lower stress on the sample containers because no aging effects caused by liquid nitrogen
  • lower consumption of liquid nitrogen


6. What is the advantage of the temperature range ≤ -130°C?


  • According to scientific studies the shelf life or survival rate of certain organic materials (e.g. cells) compared to storage at -80° C is increased significantly.
  • At -130° C the activity of the samples decreased strongly; even lower temperatures, these parameters increase only slightly, resulting in a cost-benefit ration.


7. What means a closed cooling chain?


  • Samples do not contact with the environment (humidity) during the entire handling inside ASKION C-line® equipment; this will minimize the formation of ice.
  • The temperature is constantly under -100°C (adjustable) during all handlings steps.


8. What is the consumption of liquid nitrogen?


  • hermetic storage: standby ca. 10 kg/day operating ca. 50 kg/day
  • work bench: operating ca. 20 kg/day


9. Is the ASKION C-line® medically approved?


  • Hermetic storage und work bench are also available as medical devices, confirm with European Directives class 2A applied harmonised standards 93/42.
  • „ICEBREAKER“-vials are medical devices class 2A applied harmonised standards 93/42.


10. How effect the installation on an existing laboratory system?


  • In general each single part of ASKION C-line® System can be integrated in a existing laboratory system.
  • The data connection is realized by a customized interface.


11. What services are provides ASKION as a system supplier?


  • ASKION offers expert advice, installation and service
  • technical advice for planning of the laboratory facilities
  • supplying accessories and spare parts